Journal: bioRxiv
Article Title: ATF4 and EcR interact to mediate both transcriptional activation and repression in the Drosophila fat body
doi: 10.64898/2026.03.10.710866
Figure Lengend Snippet: A) Table summarizing the overall count of differentially regulated genes in RNAseq analyses performed in isolated fat bodies where the indicated RNAi lines are driven by Dcg-GAL4 . B) Venn-diagram showing the commonalities in the differentially downregulated (left) and upregulated genes (right) in A. C-E) Counts per million (CPM) for the indicated transcripts from the RNAseq analysis in A . C and D show data for known EcR targets, E shows data for known ATF4 targets. Also see Table S1 and S2 . ****=p<0.00001; ***=p<0.0001; **=p<0.001; *=p<0.05. Only significant comparisons are shown. Statistical tests are described in the methods.
Article Snippet: Duplicate RNA samples from independent crosses were submitted for each genotype to the Plasmidsaurus RNAseq analysis pipeline.
Techniques: RNA sequencing, Isolation